Cfu Means Colony Case Study On A Biology Paper
Paper: Chromek et al. (2012) The Antimicrobial Peptide Cathelicidin Protects Mice from Escherichia coli O157:H7-Mediated Disease. PLoS One.
>>> Please, no patch-writing or copying anything. This class is heavy on plagiarism. It is critical that your responses be formulated in your own words, that you NOT copy sentences or phrases from the published paper. I uploaded the Plagiarism form, read please because policy is a little different and strict. Thank you!
>>>Instructions. READ CAREFULLY
1. Use 1 inch margins all around.
2. Use Times 12 point font, single spaced (like this page). If you use any other font or size, you will receive 0 credit. This will be enforced.
3. Do not re-write the questions on the page; simply write your answers. One and a half page maximum (you can go ó way down the second page; no more). If you use more than one page, the top line of text should start at the same point on both pages. Staple them together.
4. Format your paper in a way that is easy to follow, so it is clear to the grader what you are answering. The answer to each question (1, 2, 3, etc.) should start on a new line. Each answer must be preceded by the letter of the corresponding question (a, b, c, etc.) so the grader knows which question you are answering.
5. At the top left side of the page type: “SECTION PAPER 1, WEEK OF OCTOBER 7, 2019.” Then type the last four digits of your student ID at the top right corner of the page. I have done so above as an example. Do not write your name on the page.
6. It is important, encouraged, and assumed that you will read and discuss these papers in your study groups. Write up the answers, however, in your own words and with your own thoughts. Don’t copy each other. Do not cut and paste from the paper. Do not use “patch-writing.” Do not plagiarize.
7. Submit your paper to Turnitin using the link on Canvas (in the section paper folder). UPLOAD YOUR PAPER AS A WORD DOCUMENT! not a PDF.
8. Even though you will be answering questions regarding a few figure panels, be sure to read the entire paper, including the methods, as this is essential in helping you answer the questions.
Helpful tips:
1. Read the material and methods section of the paper for details about how the experiments were done.
2. Look on the internet or in the other textbooks online for background information on topics/reagents/techniques that you do not understand.
3. Alcian blue is a dye which binds to polysaccharides (found in mucus).
4. ad libitum indicates food available at all times with the quantity and frequency of consumption being the free choice of the animal.
5. CFU means “colony forming unit,” in other words, live bacteria. So CFU/ml means the number of live bacteria per ml. CFU/g is live bacteria per gram.
6. Hemolytic anemia is the premature destruction of red blood cells. It can occur if your red blood cells don’t contain enough hemoglobin
7. Thrombocytopenia is a low platelet count.
8. Hemolytic Uremic Syndrome is typically characterized by reduced hemoglobin levels, hemolytic anemia, thrombocytopenia, elevated creatinine levels (sign of kidney dysfunction).
9. Desquamation refers to shedding of the epithelial cells.
>>>Answer the following questions. Be sure to number your answers to correspond to each question and be sure to answer each question.
1. a. Briefly, what experiment is done in Figure 1A,B? (summarize, in 1-2 sentences)
b. What were they measuring?
c. What control(s) did they perform to make sure that the system was working correctly?
d. What results did they see?
e. How did they interpret the experiment? What is the significance?
2. a. Briefly, what experiment is done in Figure 2A,B? (summarize, in 1-2 sentences)
b. How did they do it? (summary of the method, touching on the most important points to communicate how the experiment was done)
c. Prior to generating the results in figure 2, their first attempt at this experiment failed. Explain why.
d. What were they measuring?
e. How could they be confident they were counting EHEC only, and not other bacteria in the feces?
f. How did they interpret the experiment? What is the significance?
3. a. Briefly, what experiment is done in Figure 3E,F?
b. What were they investigating?
c. What control(s) did they perform? What was their purpose?
d. What results did they see?
e. How did they interpret the experiment?
4. a. Briefly, what experiment is done in Figure 4A,B?
b. What results did they see?
c. Why was this important to include in the paper?
5. What significant medical advancement may result from continuing these investigations?